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HPLC analyses were performed using a Phenomenex Kinetex PFP column (3 × 150 mm) using water/formic acid 100/0.1 v/v (A) and acetonitrile/formic acid 100/0.1 v/v (B) as mobile phases at 0.8 ml/min flow

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Following the standard synthesis and purification process, the finished DIHEXA research material undergoes an additional preparative high-performance liquid chromatography purification stage designed to reduce residual synthesis-related and process-related impurities further