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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 × Modified Eagle’s medium alpha (alpha MEM) (Gibco), 2.8 μM hydrocortisone, 1 μg/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)