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DLS measures the hydrodynamic diameter, which includes not only the nanoparticle core but also the surrounding solvation layer, adsorbed biomolecules, and any potential agglomeration in solution[22,25,38]

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The kidneys were collected after euthanasia and after stripping off the capsule, part of the tissues were fixed using 10% formalin at 45°C for 1 h and embedded in paraffin