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A broad panel of antibodies was employed, including those for FOXO4, p53, phospho-p53, p21, p16, cleaved-Caspase3, BAX, BCL-2, testosterone synthesis enzymes (3β-HSD, CYP11A1, CYP17A1), and various SASP factors (IL-1β, IL-6, TNF-α, TGF-β)

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Carotenoids are synthesized in living organisms by phytoene synthase, which produces the 40-carbon core of carotenoid molecules, carotene desaturase, which forms double bonds between adjacent carbons, and carotene cyclase, which catalyzes the reaction to attach six-carbon rings to the ends of the 40-carbon backbone [5]